Considering emergence and maintenance of cooperative behavior, based on evolutionary game theory and network theory, an evolutionary game model which can promote cooperation was proposed. In the proposed model, time scale and selection preference were introduced simultaneously into evolutionary game. In initialization phase, players were segmented into two categories according to their time scales of the strategies. Players in one category updated their strategies in each round, while players in the other category determined wether to update their strategies according to certain probability after every round of game. In strategy updating phase, the reputation of a player was determined by his distribution to his neighbors, and all players perfered to learn the strategies of neighbors with good reputation. The simulation experimental results show that, in the proposed evolutionary game model under synergistic effect of time scale and selection preference, cooperative behavior can be maintained in the group, the players with inertia hinders the emergence of cooperation, but the irrational behavior of players can promote cooperation.